Ingredient file // Vitamin or mineral
Vitamin B2
A necessary vitamin, not an energy boost
Vitamin B2, also called riboflavin, is added to help a dog food meet its nutrient target. Dogs need it to process energy from food. Extra B2 has no demonstrated energy, coat or eye benefit for a dog already getting enough.
Role in the food Supporting nutrient- What it is
- Riboflavin, a water-soluble vitamin added in small amounts to complete a dog food's nutrient profile.
- Made from
- Glucose fermented by selected microbes
- Main job
- Meet the B2 requirement
- Typical amount
- Usually under 10 mg per kg of complete feed
- Watch for
- Diets without a nutritional adequacy statement
- Not the same as
- Vitamin B12
Straight answer
Is Vitamin B2 safe for dogs?
Read the adequacy statement before the vitamin list
Vitamin B2 near the end of a bag's ingredient list is expected because the recipe needs only a small amount. Check the nutritional adequacy statement for your dog's life stage. Treat riboflavin as part of the vitamin mix, and pass over promises of extra energy, a shinier coat or protected eyes.
- Why it is in the food
- Meet the B2 requirement
- Watch for
- Incomplete regular diets
- How common
- Listed by 44 of the 3,204 foods we track
Our read // In our catalog
In 1 of every 73 foods we track
Share of foods that list it
1%
44 of the 3,204 foods with an ingredient list on file, from 7 brands. 32 dry dog food, 8 frozen dog food, 3 wet dog food.
Each square is 1% of the catalog
Where it sits on the label
Median position 36.5. In the top five of 0 foods, so it is usually a minor ingredient.
Above the salt
Usually listed after the salt, which caps it at roughly 1% of the recipe in 100% of foods.
Price per lb
Median of the cheapest bag price across 23 priced foods, 28% below the catalog. The price is the food's, not the ingredient's.
Who leans on it
39%
of the foods that list it are from Nulo. 7 brands use it in total.
What it is
How it is made
Vitamin makers produce riboflavin deliberately through fermentation, rather than recovering it from another food process. A documented feed grade production line starts with glucose and bacterial cultures, then dries the resulting vitamin for shipment.2,8
- 01 // Source Glucose and microbes Glucose feeds selected bacterial cultures in the documented feed grade production process.
- 02 // Process Fermented The cultures produce riboflavin in a liquid that moves to the recovery stage.
- 03 // Process Separated and dried The liquid is centrifuged, dispersed and spray dried into a powder.
- 04 // Output B2 powder The finished powder is collected, sieved and packaged for blending into a vitamin premix.
What is in it
Composition, dry matter
82.7
% riboflavin
Approximate active vitamin in one tested feed grade powder. Much of the remainder is maltodextrin, which helps prepare the additive.
Why it is in the bag
A tiny but required vitamin, not filler
- Main nutrition
- Supporting nutrient
- Does a job
- Mostly filler
Makers add riboflavin so the finished food reaches the B2 minimum in dog food nutrient profiles. EFSA judges feed riboflavin effective at covering an animal's B2 requirement. Labels list ingredients by weight, and dogs need B2 in milligrams per kilogram of food, so it sits near the end. That small amount is the job, not a sign of filler.2,4,5
-
01
Meets the nutrient target
Food makers add riboflavin so the finished recipe supplies the required amount even when its other ingredients contribute varying amounts.3,4
-
02
Supplies an energy cofactor
Dogs use riboflavin in compounds that process carbohydrate, fat and protein. Meeting the requirement supports that function; adding more is not an energy treatment.1
-
03
Mixes at small scale
The vitamin is supplied as a concentrated powder. Premix makers blend vitamins with carriers so small measured amounts distribute through a complete feed.2,8
-
04
Ships concentrated
Vitamin suppliers ship concentrated material because transporting the added weight of premix carriers costs more. The carriers are blended in later.8
How much AAFCO's corrected dog food profiles set a minimum of 5.2 mg riboflavin per kilogram of dry food for growth and adult maintenance.4
Our read // The claims
What the evidence supports
Strong: controlled studies in dogs agree. Some: small or mixed dog data. Thin: mechanism or lab work only. None: nothing, or the evidence points the other way.
-
Brands say
Dogs need B2 to use energy from food
Evidence
- Strong
- Some
- Thin
- None
FEDIAF summarizes an adult dog requirement study that measured a deficiency marker and estimated a need of 66.8 micrograms per kilogram of body weight daily. That supports adequate intake, not a claim that extra B2 improves performance.1,3
-
Brands say
Extra B2 makes a dog's coat healthier
Outruns the evidence
Evidence
- Strong
- Some
- Thin
- None
A clinical nutrition chapter describes skin lesions from experimental B-vitamin deficiency and discusses dog feeding studies that added several nutrients together. Those studies cannot show that extra riboflavin alone improves an adequately fed dog's coat.6
-
Brands say
Extra B2 protects a dog's eyes
Outruns the evidence
Evidence
- Strong
- Some
- Thin
- None
A veterinary nutrition text lists cloudy corneas among signs of riboflavin deficiency in dogs. The EFSA review of an 80% feed additive assessed it as a nutrient source, not as a treatment that protects healthy eyes.1,7
-
Owners say
Fermented B2 is unsafe for dogs
Outruns the evidence
Evidence
- Strong
- Some
- Thin
- None
EFSA found one earlier fermented B2 product posed a risk because it held live cells and DNA of a modified strain carrying antibiotic resistance genes. Products from that strain later tested clean in 3 batches and were judged safe. The problem was contamination, not fermentation.2
-
Owners say
More B2 gives a healthy dog more energy
Outruns the evidence
Evidence
- Strong
- Some
- Thin
- None
Riboflavin participates in energy metabolism, but that does not mean an extra dose makes a well-fed dog livelier. EFSA assessed an 80% riboflavin additive for meeting nutritional needs, not for increasing activity or stamina.1
Straight answers
What owners ask
-
Q01
Is it just filler?
NoRiboflavin has a defined nutrient job, and dog food profiles specify a minimum amount. Maltodextrin in one feed grade powder is a carrier; it does not make the riboflavin line meaningless.2,4
-
Q02
Why is it near the end?
Small doseIngredients are listed by weight. Dogs need riboflavin in milligrams per kilogram of food, so its place near the end is expected and says little about the recipe's quality.4,5
-
Q03
Does my dog need extra B2?
Usually noA complete and balanced food for your dog's life stage is meant to supply required nutrients. Riboflavin appearing on its ingredient list is not a reason to add a separate supplement.3,5
Who uses it
Brands, best sellers and the company it keeps
Top brands, by foods that list it
Foods that list it / all the brand's recipes we track.
Usually listed with, share of the foods that list it
- Flaxseed 64%
- Barley 55%
- Chicken Fat 52%
- Brown Rice 48%
- Dried Plain Beet Pulp 48%
- Chicken Meal 36%
- Rice Bran 30%
- Sweet Potatoes 27%
- Sunflower Oil 25%
- Fish Meal 25%
- Natural Flavor 25%
- Carrots 20%
On the shelf
Foods that list Vitamin B2
44 foods in our catalog list it, highest on the label first.
Earthborn HolisticEarthborn Holistic Venture Turkey Meal & Pumpkin Grain-Free Dry Dog Food
PedigreePedigree Adult, Roasted Lamb & Vegetable Dry Dog Food
Earthborn HolisticEarthborn Holistic Meadow Feast Grain-Free Dry Dog Food
Northwest NaturalsNorthwest Naturals Fruits & Veggies Dog Food Topper
IamsIams Proactive Health Sensitive Skin & Stomach Adult Salmon Dry Dog Food
NuloNulo FreeStyle Signature Stews Small Breed Beef, Beef Liver & Kale Recipe Grain-Free Wet Dog Food
NuloNulo FreeStyle Signature Stews Small Breed Lamb & Pumpkin Recipe Grain-Free Wet Dog Food
NuloNulo Culinary Harvest Puppy Chicken & Whitefish Recipe Dry Dog Food
NuloNulo Culinary Harvest Chicken & Whitefish Recipe Dry Dog Food
WellnessWellness CORE+ Wholesome Grains Sensitive Skin Stomach Salmon & Rice Dry Dog Food
NuloNulo Gently-Cooked Meals Beef & Sweet Potato Recipe Frozen Dog Food
NuloNulo Gently-Cooked Meals Salmon & Brown Rice Recipe Frozen Dog Food Show 32 more
Earthborn HolisticEarthborn Holistic Primitive Natural Grain-Free Dry Dog Food
Earthborn HolisticEarthborn Holistic Coastal Catch Grain-Free Dry Dog Food
NuloNulo FreeStyle Signature Stews Small Breed Tuna, Pollock & Salmon Recipe Grain-Free Wet Dog Food
NuloNulo Culinary Harvest Turkey & Herring Recipe Dry Dog Food
NuloNulo Culinary Harvest Beef, Pork & Lamb Recipe Dry Dog Food
NuloNulo Culinary Harvest Small Breed Beef, Pork & Lamb Recipe Dry Dog Food
WellnessWellness Core RawRev Wholesome Grains Ocean + 100% Raw Fish Dry Dog Food
Blue SealBlue Seal Native Level 3 Chicken Meal & Rice Dry Dog Food
NuloNulo Gently-Cooked Meals Chicken & Quinoa Recipe Frozen Dog Food
Blue SealBlue Seal Native Level 2.5 Dry Dog Food
NuloNulo Gently-Cooked Meals Small Breed Beef & Sweet Potato Recipe Frozen Dog Food
WellnessWellness Core Ocean Grain-Free Dry Dog Food
Blue SealBlue Seal Native Level 2 Chicken Meal & Rice Dry Dog Food
Blue SealBlue Seal Native Level 1 Lamb Meal & Rice Formula Dry Dog Food
NuloNulo Gently-Cooked Meals Small Breed Salmon & Brown Rice Recipe Frozen Dog Food
NuloNulo Gently-Cooked Meals Small Breed Chicken & Quinoa Recipe Frozen Dog Food
NuloNulo Gently-Cooked Meals Lamb & Apple Recipe Frozen Dog Food
NuloNulo MedalSeries Baked & Coated Salmon, Chicken & Turkey Recipe Grain-Free Dry Dog Food
WellnessWellness Core RawRev Wholesome Grains Small Breed + 100% Raw Turkey Dry Dog Food
WellnessWellness Core RawRev Wholesome Grains Original + 100% Raw Turkey Dry Dog Food
WellnessWellness Core Wholesome Grains Original Dry Dog Food
NuloNulo Gently-Cooked Meals Duck, Chicken & Quinoa Recipe Frozen Dog Food
WellnessWellness Core Reduced Fat Grain-Free Dry Dog Food
Blue SealBlue Seal EnTrust Healthy Weight Formula Dry Dog Food
Blue SealBlue Seal EnTrust Small Breed Formula Dry Dog Food
Blue SealBlue Seal EnTrust Healthy Adult Formula - Pork & Barley Recipe Dry Dog Food
Blue SealBlue Seal EnTrust Healthy Senior Formula Dry Dog Food
Blue SealBlue Seal EnTrust Sensitive Stomach & Skin Formula Dry Dog Food
Blue SealBlue Seal EnTrust Healthy Adult Formula - Lamb & Rice Recipe Dry Dog Food
Blue SealBlue Seal Entrust Adult Chicken Meal & Barley Recipe Dry Dog Food
Blue SealBlue Seal Entrust Large Breed Puppy Chicken Meal & Barley Recipe Dry Dog Food
Blue SealBlue Seal Entrust Puppy Chicken Meal & Barley Recipe Dry Dog Food Sources
What we read
Checked October 22, 2026.
- 1 Safety and efficacy of vitamin B2 (riboflavin) produced by Ashbya gossypii DSM 23096 for all animal species based on a dossier submitted by BASF SE
- 2 Safety and efficacy of feed additives consisting of Vitamin B2 (98%) and Vitamin B2 (80%) as riboflavin produced by Bacillus subtilis KCCM 10445 for all animal species (Hubei Guangji Pharmaceutical Co. Ltd.)
- 3 FEDIAF Nutritional Guidelines for Complete and Complementary Pet Food for Cats and Dogs
- 4 Pet Food Committee Minutes, AAFCO Dog and Cat Food Nutrient Profiles Errors in 2016 Official Publication
- 5 Reading Labels
- 6 Small Animal Clinical Nutrition, 5th Edition, Chapter 32
- 7 Applied Veterinary Clinical Nutrition
- 8 Understanding the vitamin supply chain and relative risk of transmission of foreign animal diseases