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Ingredient file // Vitamin or mineral

Vitamin B2

A necessary vitamin, not an energy boost

Vitamin B2, also called riboflavin, is added to help a dog food meet its nutrient target. Dogs need it to process energy from food. Extra B2 has no demonstrated energy, coat or eye benefit for a dog already getting enough.

Role in the food Supporting nutrient
What it is
Riboflavin, a water-soluble vitamin added in small amounts to complete a dog food's nutrient profile.
Made from
Glucose fermented by selected microbes
Main job
Meet the B2 requirement
Typical amount
Usually under 10 mg per kg of complete feed
Watch for
Diets without a nutritional adequacy statement
Not the same as
Vitamin B12

Straight answer

Is Vitamin B2 safe for dogs?

Read the adequacy statement before the vitamin list

Vitamin B2 near the end of a bag's ingredient list is expected because the recipe needs only a small amount. Check the nutritional adequacy statement for your dog's life stage. Treat riboflavin as part of the vitamin mix, and pass over promises of extra energy, a shinier coat or protected eyes.

Why it is in the food
Meet the B2 requirement

Our read // In our catalog

In 1 of every 73 foods we track

Share of foods that list it

1%

44 of the 3,204 foods with an ingredient list on file, from 7 brands. 32 dry dog food, 8 frozen dog food, 3 wet dog food.

Each square is 1% of the catalog

Where it sits on the label

Median position 36.5. In the top five of 0 foods, so it is usually a minor ingredient.

Top five List position, heaviest first

Above the salt

0%

Usually listed after the salt, which caps it at roughly 1% of the recipe in 100% of foods.

Price per lb

Foods with it $2.34
All foods $3.25

Median of the cheapest bag price across 23 priced foods, 28% below the catalog. The price is the food's, not the ingredient's.

Who leans on it

39%

of the foods that list it are from Nulo. 7 brands use it in total.

What it is

How it is made

Vitamin makers produce riboflavin deliberately through fermentation, rather than recovering it from another food process. A documented feed grade production line starts with glucose and bacterial cultures, then dries the resulting vitamin for shipment.2,8

  1. 01 // Source Glucose and microbes Glucose feeds selected bacterial cultures in the documented feed grade production process.
  2. 02 // Process Fermented The cultures produce riboflavin in a liquid that moves to the recovery stage.
  3. 03 // Process Separated and dried The liquid is centrifuged, dispersed and spray dried into a powder.
  4. 04 // Output B2 powder The finished powder is collected, sieved and packaged for blending into a vitamin premix.

What is in it

Composition, dry matter

82.7

% riboflavin

Approximate active vitamin in one tested feed grade powder. Much of the remainder is maltodextrin, which helps prepare the additive.

Riboflavin 81.3-84.2%2
Maltodextrin 15.7-16.9%2
Ash 0.1-1%2

These approximate dry matter ranges convert published as-sold measurements using the reported drying-loss range. The batch endpoints were not paired, and other riboflavin powders use different amounts of carrier.2

Why it is in the bag

A tiny but required vitamin, not filler

  1. Main nutrition
  2. Supporting nutrient
  3. Does a job
  4. Mostly filler

Makers add riboflavin so the finished food reaches the B2 minimum in dog food nutrient profiles. EFSA judges feed riboflavin effective at covering an animal's B2 requirement. Labels list ingredients by weight, and dogs need B2 in milligrams per kilogram of food, so it sits near the end. That small amount is the job, not a sign of filler.2,4,5

  1. 01

    Meets the nutrient target

    Food makers add riboflavin so the finished recipe supplies the required amount even when its other ingredients contribute varying amounts.3,4

  2. 02

    Supplies an energy cofactor

    Dogs use riboflavin in compounds that process carbohydrate, fat and protein. Meeting the requirement supports that function; adding more is not an energy treatment.1

  3. 03

    Mixes at small scale

    The vitamin is supplied as a concentrated powder. Premix makers blend vitamins with carriers so small measured amounts distribute through a complete feed.2,8

  4. 04

    Ships concentrated

    Vitamin suppliers ship concentrated material because transporting the added weight of premix carriers costs more. The carriers are blended in later.8

How much AAFCO's corrected dog food profiles set a minimum of 5.2 mg riboflavin per kilogram of dry food for growth and adult maintenance.4

Our read // The claims

What the evidence supports

Strong: controlled studies in dogs agree. Some: small or mixed dog data. Thin: mechanism or lab work only. None: nothing, or the evidence points the other way.

  1. Brands say

    Dogs need B2 to use energy from food

    Evidence

    1. Strong
    2. Some
    3. Thin
    4. None

    FEDIAF summarizes an adult dog requirement study that measured a deficiency marker and estimated a need of 66.8 micrograms per kilogram of body weight daily. That supports adequate intake, not a claim that extra B2 improves performance.1,3

  2. Brands say

    Extra B2 makes a dog's coat healthier

    Outruns the evidence

    Evidence

    1. Strong
    2. Some
    3. Thin
    4. None

    A clinical nutrition chapter describes skin lesions from experimental B-vitamin deficiency and discusses dog feeding studies that added several nutrients together. Those studies cannot show that extra riboflavin alone improves an adequately fed dog's coat.6

  3. Brands say

    Extra B2 protects a dog's eyes

    Outruns the evidence

    Evidence

    1. Strong
    2. Some
    3. Thin
    4. None

    A veterinary nutrition text lists cloudy corneas among signs of riboflavin deficiency in dogs. The EFSA review of an 80% feed additive assessed it as a nutrient source, not as a treatment that protects healthy eyes.1,7

  4. Owners say

    Fermented B2 is unsafe for dogs

    Outruns the evidence

    Evidence

    1. Strong
    2. Some
    3. Thin
    4. None

    EFSA found one earlier fermented B2 product posed a risk because it held live cells and DNA of a modified strain carrying antibiotic resistance genes. Products from that strain later tested clean in 3 batches and were judged safe. The problem was contamination, not fermentation.2

  5. Owners say

    More B2 gives a healthy dog more energy

    Outruns the evidence

    Evidence

    1. Strong
    2. Some
    3. Thin
    4. None

    Riboflavin participates in energy metabolism, but that does not mean an extra dose makes a well-fed dog livelier. EFSA assessed an 80% riboflavin additive for meeting nutritional needs, not for increasing activity or stamina.1

Straight answers

What owners ask

  1. Q01

    Is it just filler?

    No

    Riboflavin has a defined nutrient job, and dog food profiles specify a minimum amount. Maltodextrin in one feed grade powder is a carrier; it does not make the riboflavin line meaningless.2,4

  2. Q02

    Why is it near the end?

    Small dose

    Ingredients are listed by weight. Dogs need riboflavin in milligrams per kilogram of food, so its place near the end is expected and says little about the recipe's quality.4,5

  3. Q03

    Does my dog need extra B2?

    Usually no

    A complete and balanced food for your dog's life stage is meant to supply required nutrients. Riboflavin appearing on its ingredient list is not a reason to add a separate supplement.3,5

Who uses it

Brands, best sellers and the company it keeps

Top brands, by foods that list it

  1. Nulo 17 / 117
  2. Blue Seal 13 / 19
  3. Wellness 7 / 118
  4. Earthborn Holistic 4 / 28
  5. Northwest Naturals 1 / 5
  6. Pedigree 1 / 42
  7. Iams 1 / 31

Foods that list it / all the brand's recipes we track.

Usually listed with, share of the foods that list it

  1. Flaxseed 64%
  2. Barley 55%
  3. Chicken Fat 52%
  4. Brown Rice 48%
  5. Dried Plain Beet Pulp 48%
  6. Chicken Meal 36%
  7. Rice Bran 30%
  8. Sweet Potatoes 27%
  9. Sunflower Oil 25%
  10. Fish Meal 25%
  11. Natural Flavor 25%
  12. Carrots 20%

On the shelf

Foods that list Vitamin B2

44 foods in our catalog list it, highest on the label first.

Show 32 more
Earthborn Holistic Primitive Natural Grain-Free Dry Dog FoodEarthborn HolisticEarthborn Holistic Primitive Natural Grain-Free Dry Dog FoodDry // No. 25 on the labelEarthborn Holistic Coastal Catch Grain-Free Dry Dog FoodEarthborn HolisticEarthborn Holistic Coastal Catch Grain-Free Dry Dog FoodDry // No. 27 on the labelNulo FreeStyle Signature Stews Small Breed Tuna, Pollock & Salmon Recipe Grain-Free Wet Dog FoodNuloNulo FreeStyle Signature Stews Small Breed Tuna, Pollock & Salmon Recipe Grain-Free Wet Dog FoodWet // No. 30 on the labelNulo Culinary Harvest Turkey & Herring Recipe Dry Dog FoodNuloNulo Culinary Harvest Turkey & Herring Recipe Dry Dog FoodDry // No. 31 on the labelNulo Culinary Harvest Beef, Pork & Lamb Recipe Dry Dog FoodNuloNulo Culinary Harvest Beef, Pork & Lamb Recipe Dry Dog FoodDry // No. 32 on the labelNulo Culinary Harvest Small Breed Beef, Pork & Lamb Recipe Dry Dog FoodNuloNulo Culinary Harvest Small Breed Beef, Pork & Lamb Recipe Dry Dog FoodDry // No. 32 on the labelWellness Core RawRev Wholesome Grains Ocean + 100% Raw Fish Dry Dog FoodWellnessWellness Core RawRev Wholesome Grains Ocean + 100% Raw Fish Dry Dog FoodDry // No. 35 on the labelBlue Seal Native Level 3 Chicken Meal & Rice Dry Dog FoodBlue SealBlue Seal Native Level 3 Chicken Meal & Rice Dry Dog FoodDry // No. 35 on the labelNulo Gently-Cooked Meals Chicken & Quinoa Recipe Frozen Dog FoodNuloNulo Gently-Cooked Meals Chicken & Quinoa Recipe Frozen Dog FoodFrozen // No. 36 on the labelBlue Seal Native Level 2.5 Dry Dog FoodBlue SealBlue Seal Native Level 2.5 Dry Dog FoodDry // No. 36 on the labelNulo Gently-Cooked Meals Small Breed Beef & Sweet Potato Recipe Frozen Dog FoodNuloNulo Gently-Cooked Meals Small Breed Beef & Sweet Potato Recipe Frozen Dog FoodFrozen // No. 37 on the labelWellness Core Ocean Grain-Free Dry Dog FoodWellnessWellness Core Ocean Grain-Free Dry Dog FoodDry // No. 37 on the labelBlue Seal Native Level 2 Chicken Meal & Rice Dry Dog FoodBlue SealBlue Seal Native Level 2 Chicken Meal & Rice Dry Dog FoodDry // No. 37 on the labelBlue Seal Native Level 1 Lamb Meal & Rice Formula Dry Dog FoodBlue SealBlue Seal Native Level 1 Lamb Meal & Rice Formula Dry Dog FoodDry // No. 37 on the labelNulo Gently-Cooked Meals Small Breed Salmon & Brown Rice Recipe Frozen Dog FoodNuloNulo Gently-Cooked Meals Small Breed Salmon & Brown Rice Recipe Frozen Dog FoodFrozen // No. 38 on the labelNulo Gently-Cooked Meals Small Breed Chicken & Quinoa Recipe Frozen Dog FoodNuloNulo Gently-Cooked Meals Small Breed Chicken & Quinoa Recipe Frozen Dog FoodFrozen // No. 38 on the labelNulo Gently-Cooked Meals Lamb & Apple Recipe Frozen Dog FoodNuloNulo Gently-Cooked Meals Lamb & Apple Recipe Frozen Dog FoodFrozen // No. 38 on the labelNulo MedalSeries Baked & Coated Salmon, Chicken & Turkey Recipe Grain-Free Dry Dog FoodNuloNulo MedalSeries Baked & Coated Salmon, Chicken & Turkey Recipe Grain-Free Dry Dog FoodDry // No. 38 on the labelWellness Core RawRev Wholesome Grains Small Breed + 100% Raw Turkey Dry Dog FoodWellnessWellness Core RawRev Wholesome Grains Small Breed + 100% Raw Turkey Dry Dog FoodDry // No. 38 on the labelWellness Core RawRev Wholesome Grains Original + 100% Raw Turkey Dry Dog FoodWellnessWellness Core RawRev Wholesome Grains Original + 100% Raw Turkey Dry Dog FoodDry // No. 38 on the labelWellness Core Wholesome Grains Original Dry Dog FoodWellnessWellness Core Wholesome Grains Original Dry Dog FoodDry // No. 38 on the labelNulo Gently-Cooked Meals Duck, Chicken & Quinoa Recipe Frozen Dog FoodNuloNulo Gently-Cooked Meals Duck, Chicken & Quinoa Recipe Frozen Dog FoodFrozen // No. 39 on the labelWellness Core Reduced Fat Grain-Free Dry Dog FoodWellnessWellness Core Reduced Fat Grain-Free Dry Dog FoodDry // No. 40 on the labelBlue Seal EnTrust Healthy Weight Formula Dry Dog FoodBlue SealBlue Seal EnTrust Healthy Weight Formula Dry Dog FoodDry // No. 52 on the labelBlue Seal EnTrust Small Breed Formula Dry Dog FoodBlue SealBlue Seal EnTrust Small Breed Formula Dry Dog FoodDry // No. 52 on the labelBlue Seal EnTrust Healthy Adult Formula - Pork & Barley Recipe Dry Dog FoodBlue SealBlue Seal EnTrust Healthy Adult Formula - Pork & Barley Recipe Dry Dog FoodDry // No. 52 on the labelBlue Seal EnTrust Healthy Senior Formula Dry Dog FoodBlue SealBlue Seal EnTrust Healthy Senior Formula Dry Dog FoodDry // No. 53 on the labelBlue Seal EnTrust Sensitive Stomach & Skin Formula Dry Dog FoodBlue SealBlue Seal EnTrust Sensitive Stomach & Skin Formula Dry Dog FoodDry // No. 53 on the labelBlue Seal EnTrust Healthy Adult Formula - Lamb & Rice Recipe Dry Dog FoodBlue SealBlue Seal EnTrust Healthy Adult Formula - Lamb & Rice Recipe Dry Dog FoodDry // No. 54 on the labelBlue Seal Entrust Adult Chicken Meal & Barley Recipe Dry Dog FoodBlue SealBlue Seal Entrust Adult Chicken Meal & Barley Recipe Dry Dog FoodDry // No. 54 on the labelBlue Seal Entrust Large Breed Puppy Chicken Meal & Barley Recipe Dry Dog FoodBlue SealBlue Seal Entrust Large Breed Puppy Chicken Meal & Barley Recipe Dry Dog FoodDry // No. 54 on the labelBlue Seal Entrust Puppy Chicken Meal & Barley Recipe Dry Dog FoodBlue SealBlue Seal Entrust Puppy Chicken Meal & Barley Recipe Dry Dog FoodDry // No. 54 on the label

Sources

What we read

Checked October 22, 2026.

  1. 1 Safety and efficacy of vitamin B2 (riboflavin) produced by Ashbya gossypii DSM 23096 for all animal species based on a dossier submitted by BASF SE EFSA Journal, 2018 // Journal
  2. 2 Safety and efficacy of feed additives consisting of Vitamin B2 (98%) and Vitamin B2 (80%) as riboflavin produced by Bacillus subtilis KCCM 10445 for all animal species (Hubei Guangji Pharmaceutical Co. Ltd.) EFSA Journal, 2021 // Journal
  3. 3 FEDIAF Nutritional Guidelines for Complete and Complementary Pet Food for Cats and Dogs FEDIAF, 2021 // Industry
  4. 4 Pet Food Committee Minutes, AAFCO Dog and Cat Food Nutrient Profiles Errors in 2016 Official Publication AAFCO, 2016 // Regulator
  5. 5 Reading Labels AAFCO, n.d. // Regulator
  6. 6 Small Animal Clinical Nutrition, 5th Edition, Chapter 32 Mark Morris Institute, 2010 // Book
  7. 7 Applied Veterinary Clinical Nutrition Wiley-Blackwell, 2012 // Book
  8. 8 Understanding the vitamin supply chain and relative risk of transmission of foreign animal diseases University of Minnesota, 2019 // University
An ingredient on its own tells you very little. What matters is how much of it there is and where it sits in the list. That is why we weigh the top five rather than reacting to any single name further down. Back to the wiki

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